H2 Blue – Hydrogen Test Reagent (10mL)


Measure the concentration of dissolved hydrogen gas (H2) in water in mg/L (parts per million / ppm).


H2 Blue – Hydrogen Test Reagent (10mL)

H2 Blue is a blue reagent that reacts with water containing dissolved hydrogen gas, and turns “clear” when the dissolved H2 concentration is at least 0.1mg/L (ppm).

For more information about Hydrogen Water, please read here.

With H2 Blue, you can:

  • Measure the concentration of dissolved hydrogen gas (H2) in water in mg/L (parts per million / ppm).
  • Measure dissolved hydrogen gas in water produced by a variety of H2 water-generating technologies including: Alkaline ionizers, H2 water filters, Hydrogen Infusion Machines (HIM’s), Tablets, Sticks, Bubblers, Reactor Cartridges, etc.
  • Insure that your H2 device is operating at peak performance by monitoring the level of dissolved hydrogen every month
  • Great for demonstrating the presence of dissolved H2 – simply place a few drops in any H2 water and watch them turn clear (indicating the presence of dissolved hydrogen)
  • Accurately test H2 water in the pH range of 4 – 10

How to use H2 Blue, please read here.

What is “PPM”?

The ppm is an acronym for “parts per million”, a unit of measure that describes how much of one substance (called the “solute”) is dissolved in a sample of water (called the “solvent”). This is a measurement of concentration (or density), which can often be helpful in our daily lives. The ppm can be used to measure the concentration of many substances, such as the minerals in our drinking water or the oxygen in a fish aquarium. Although the ppm is commonly used for concentration measurements, it only provides us with a ratio of the solute’s mass to the mass of the water, without specifying the water’s total volume. But, more often we need to know the total amount of solute dissolved in the water. This is important, for example, when determining the therapeutic dose for a medicine or the ingested level of a toxin. Without including the size of the container we are testing, which tells us the amount of water our solute is dissolved in, the ppm ratio by itself does not tell us how much solute the water contains. To convey this information, scientists use a more appropriate unit of measure, one which specifies the solute’s concentration in units of “mass per unit volume”. The unit commonly used is the “milligram per liter”, abbreviated mg/L. The mg/L always references the solute’s mass relative to a fixed volume, one liter.

Note: Frequently, the ppb (parts per billion) is used to measure solute concentration. Although not specifically addressed here, 1 ppm = 1000 ppb.

​​​​How are ppm and mg/L related?

​We can think of 1 ppm as “1 part of a substance (solute) dissolved in 1 million parts of a solution” (in this case the solute is H2 (hydrogen) gas and the solution is water). So, what is a “part”? “Part” represents a unit of measure, in our case the “milligram” (mg). Therefore, 1 milligram of H2 (“1 part”) dissolved in 1 million milligrams of water (“1 million parts”) is “one part per million”. And, since 1 liter of water happens to weigh 1 million milligrams, 1 ppm is equal to 1 mg/L (for dilute concentrations).

Note: This is only true when comparing units of mass, not when comparing volumes, # of moles, or # of molecules.

What does the measurement tell me about my hydrogen water?

When using H2Blue to measure a sample of hydrogen water, each drop represents 0.1mg/L of dissolved H2 gas. By adding together the total number of drops required to reach the titration endpoint (the point at which the next drop does not turn clear, but remains blue), the dissolved H2 level in the sample can be calculated. Because the results of each measurement done with H2Blue are expressed in mg/L, it is important to understand how the measurement tells us how much H2 is in our water. What we really want to know is “how much hydrogen will I ingest if I drink the entire contents of the container from which the test sample was taken”? How the results are interpreted depends, in part, upon how the hydrogen water was originally produced.​

Packaging & Storage

The H2 Blue bottle contains 10 mL of reagent, enough to perform approximately 50 tests (depending on your average H2 levels). Since the bottle capacity is approximately 13 mL, it will not appear to be completely full when received (bottles are filled using precision dispensing equipment). H2 Blue comes in an LDPE plastic bottle with a child-resistant cap. The H2 Blue bottle comes stored in a plastic graduated beaker with a screw cap. The beaker is used to measure the precise volume of hydrogen water (6 mL) required for accurate testing of dissolved H2 levels. H2 Blue ​should be stored in a cool, dry place, and should be used within 12 months of purchase.

H2 Blue is a laboratory-grade test reagent containing compounds not intended to be taken internally…keep out of reach of children, and store in a cool, dry location. Under extremely low temperatures, H2 Blue formula may freeze. If this occurs, allow to thaw at room temperature for 8 hours before using. DO NOT accelerate thawing by using a microwave oven or hot water!

NOTE: H2 Blue contains methylene blue, a compound used as a biological tissue dye. Therefore, H2 Blue can stain any surface it touches (possibly permanently, depending on porosity). It is recommended that skin, counter-tops, floors, clothing and other surrounding surfaces be protected from inadvertent splashing during use.  The user should wear safety glasses to protect eyes from splashing. Use of a mild abrasive cleanser will usually remove stains from most surfaces. Use soap & water to remove from skin.